File:Summary of SAGE.svg
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ཐོག་མའི་ཡིག་ཆ། (SVG file, nominally ༩༣༨ × ༧༥༦ pixels, file size: ༡༨༠ KB)
བསྡུས་དོན།
| Description |
English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In SAGE, the ds-cDNA is digested by restriction enzymes (at location ‘X’ and ‘X’+11) to produce 11-nucleotide ‘digitag’ fragments. These digitags are concatenated and sequences using long-read sanger sequencing (different shades of blue indicate digitags from different genes). The sequences are deconvoluted to find the occurrence number of each digitag. Digitag can be used to report on transcription of the gene that the digitag came from is known.[1] |
| Date | |
| Source | Own work |
| Author | Thomas Shafee |
| Other versions |
Also in series:
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གན་རྒྱ་ནང་ཞུགས་པ།
I, the copyright holder of this work, hereby publish it under the following license:
This file is licensed under the Creative Commons Attribution 4.0 International license.
- You are free:
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- ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.
ཡིག་ཆའི་ལོ་རྒྱུས།
ཟླ་ཚེས་/དུས་ཚོད་གནུན་ཏེ་རྩོམ་ཡིག་ལ་ལྟ་བ།
| ཚེས་གྲངས། / དུས་ཚོད། | བསྡུས་དོན། | ཚད། | སྤྱོད་མི། | བསམ་ཚུལ། | |
|---|---|---|---|---|---|
| ད་ལྟ། | ༡༢:༢༡, ༡༣ ཟླ་གཉིས་པ། ༢༠༡༧ | | ༩༣༨ × ༧༥༦ (༡༨༠ KB) | Evolution and evolvability | text as paths to avoid artefacts |
| ༡༠:༤༨, ༡༣ ཟླ་གཉིས་པ། ༢༠༡༧ |  | ༩༣༨ × ༧༥༦ (༡༠༤ KB) | Evolution and evolvability | update sequence text to 'sans-serif' open font for more robust rendering | |
| ༠༥:༢༧, ༧ ཟླ་བཅུ་བ། ༢༠༡༦ |  | ༩༣༨ × ༧༥༦ (༡༠༤ KB) | Evolution and evolvability | ce | |
| ༠༥:༢༣, ༧ ཟླ་བཅུ་བ། ༢༠༡༦ |  | ༩༣༨ × ༧༥༦ (༡༠༢ KB) | Evolution and evolvability | ce | |
| ༠༥:༡༦, ༧ ཟླ་བཅུ་བ། ༢༠༡༦ |  | ༩༣༨ × ༧༥༦ (༡༠༤ KB) | Evolution and evolvability | digitag-->tag | |
| ༠༥:༠༨, ༥ ཟླ་བཅུ་བ། ༢༠༡༦ |  | ༡,༠༢༠ × ༨༢༡ (༡༠༦ KB) | Evolution and evolvability | User created page with UploadWizard |
ཡིག་ཆ་བེད་སྤྱོད་ཁུལ།
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